Clinical and Experimental Pediatrics

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Original Article

Outcome after relapse in childhood and adolescent osteosarcoma : single institution experience in Korea

Yun-Jeong Lee, Hyun-Jae Lee, Dong-Ho Kim, Jung-Sub Lim, Jun-Ha Lee, Kyung-Duk Park, Dae-Geun Jeon, Soo-Yong Lee

Clin Exp Pediatr. 2008;51(1):78-83. Published online January 15, 2008

Purpose : Osteosarcoma is the most common primary malignant bone tumor in children and adolescents. Although survival rate of osteosarcoma patients has markedly improved, about 20-30% of patients still have a relapse. This study was aimed to find factors that influence postrelapse survival of osteosarcoma in childhood and adolescents. Methods : Between 1985 and 2004, of 461 patients who were diagnosed...

The optimal conditions to improve retrovirus-mediated transduction efficiency to NIH 3T3 cells

Jun Ah Lee, Kang-Min Lee, Hyun Jae Lee, Yun Jeong Lee, Dong Ho Kim, Jung Sub Lim, Kyung-Duk Park

Clin Exp Pediatr. 2007;50(10):1011-1017. Published online October 15, 2007

Purpose : We tried to assess the optimal conditions to improve low transduction efficiency and their effect on target cells. Methods : Cultured NIH 3T3 cells were incubated with retroviral vectors bearing an enhanced green fluorescent protein (eGFP) gene. We varied the ratio of viral vectors to target cells (1:1-1:8) and the number of transfections (X1, X2), and compared transduction...

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