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Original Article

Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction

Jong Gyun Ahn, Seong Yeol Choi, Dong Soo Kim, Ki Hwan Kim

Clin Exp Pediatr. 2012;55(11):424-429. Published online November 23, 2012

Crossref 16

Purpose

Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae-positive results.

Methods

Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital...

DOI: https://doi.org/10.3345/kjp.2012.55.11.424

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