| Cardioprotective Effects of Minocycline in Rat Myocardiac Cell Culture Induced by Hypoxia |
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Bum Seok Yang1, Hye Jin Park1, Hong Tae Kim2, Jong Won Lee3, Dong Suk Lee4, Un Seok Nho5, Hai Lee Chung1, Woo Taek Kim1 |
1Department of Pediatrics, School of Medicine, Catholic University of Daegu, Daegu, Korea
2Department of Anatomy, School of Medicine, Catholic University of Daegu, Daegu, Korea
3Department of Biochemistry, School of Medicine, Catholic University of Daegu, Daegu, Korea
4Department of Pediatrics, School of Medicine, Dong-guk University, Kyung-Ju, Korea
5Chilgok-Hanmaum-Yeunhap Pediatric Clinic, Daegu, Korea |
| 저산소 상태로 유도된 백서 심근세포 배양에서 Minocycline의 심근보호 효과 |
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양범석1, 박혜진1, 김홍태2, 이종원3, 이동석4, 노은석5, 정혜리1, 김우택1 |
1대구가톨릭대학교 의과대학 소아과학교실
2대구가톨릭대학교 의과대학 해부학교실
3대구가톨릭대학교 의과대학 생화학교실
4동국대학교 의과대학 소아과학교실
5칠곡 한마음연합소아과의원 |
Correspondence:
Woo Taek Kim, Email: wootykim@cu.ac.kr
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| Abstract |
Purpose
: Minocycline appears to reduce the mortality rate of patients with myocardial infarction. Therefore, the cardioprotective effects of minocycline through reduction of apoptosis were studied in a rat myocardiac cell culture induced by hypoxia.
Methods
: Cultured cells from the hearts of Sprague-Dawley rats were divided into two sets of groups : normoxia groups treated with 5% O2 and hypoxia groups treated with 1% O2. After several days of incubation, the control groups were not treated with minocycline, while the sample groups were treated with 1 g/mL of minocycline. The damaged cells were observed under a microscope, while apoptosis was detected using a Tdt-mediated dUTP nick and labeling(TUNEL) assay control- stained with 4'-6'-diamidino-2-phenylindole-2HCl(DAPI).
Results
: In normoxia, the control group showed a statistically significant difference from the sample group treated with 1 g/mL of minocycline. In addition, in hypoxia, there was also a statistically significant difference between the two groups. When comparing the normoxia and hypoxia groups to each other, there was a statistically significant difference between the control groups and sample groups.
Conclusion
: Minocycline was found to be protective for heart in normoxia and in hypoxia in the model of rat myocardiac cell culture. |
| Key Words:
Minocycline, Hypoxia, Myocardial infarction, Myocardiac cells, Cultured |
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