| Neuroprotective Effects of Minocycline in
Rat Brain Cortical Cell Culture Induced by Hypoxia |
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Kyung A Ha1, Bum Seok Yang1, Jin Kyung Kim1, Hong Tae Kim2, Sung Jin Ha3, Jong Won Lee3, Hai Lee Chung1, Woo Taek Kim1 |
1Department of Pediatrics, School of Medicine, The Catholic University of Korea, Daegu, Korea
2Department of Anatomy, School of Medicine, The Catholic University of Korea, Daegu, Korea
3Department of Biochemistry, School of Medicine, The Catholic University of Korea, Daegu, Korea |
| 저산소 상태로 유도된 백서 뇌세포 배양에서Minocycline의 뇌보호 효과 |
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하경아1, 양범석1, 김진경1, 김홍태2, 하성진3, 이종원3, 정혜리1, 김우택1 |
1대구가톨릭대학교 의과대학 소아과학교실
2대구가톨릭대학교 의과대학 해부학교실
3대구가톨릭대학교 의과대학 생화학교실 |
Correspondence:
Woo Taek Kim, Email: wootykim@cu.ac.kr
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| Abstract |
Purpose
: In vivo, minocycline appears to be neuroprotective. Thus, the neuroprotective effects of minocycline were studied in a rat brain cortical cell culture induced by hypoxia.
Methods
: Cultured cells from the brains of Sprague-Dawley rats were divided into two sets of groups : normoxia groups treated with 5% CO2 and hypoxia groups treated with 1% CO2. After several days of incubation, the control groups were not treated with minocycline, while the sample groups were treated with either 1 or 10 g/mL of minocycline. The damaged cells were observed under a microscope, while apoptosis was detected using a TUNEL assay control-stained with DAPI.
Results
: Among the normoxia groups, the control and sample groups treated with 1 and 10 g/mL of minocycline were all statistically significantly different from each other. Meanwhile, among the hypoxia groups, although the control was significantly different from the sample groups, there was no statistically significant difference between the sample groups. When comparing the normoxia and hypoxia groups, there was a statistically significant difference between the control groups and sample groups treated with 1 g/mL of minocycline, yet no significant difference between the sample groups treated with 10 g/mL of minocycline.
Conclusion
: Minocycline was found to be neuroprotective in normoxia and hypoxia induced rat brain cortical cell cultures. |
| Key Words:
Minocycline, Hypoxia, Brain, Culture |
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