Korean Journal of Pediatrics 2009;52(5):588-593.
Published online May 15, 2009.
Effect of growth hormone on neuronal death in hippocampal slice cultures of neonatal rats exposed to oxygen-glucose deprivation
Kyung Sik Hong1, Jihui Kang1, Myeung Ju Kim2, Jeesuk Yu1, Young Pyo Chang1
1Departments of Pediatrics, College of Medicine, Dankook University, Cheonan, Korea
2Departments of Anatomy, College of Medicine, Dankook University, Cheonan, Korea
신생 흰쥐 해마 절편 배양에서 산소-포도당 박탈에 의한 신경 세포 사망에 대한 성장호르몬의 효과
홍경식1, 강지희1, 김명주2, 유지숙1, 장영표1
1단국대학교 의과대학 소아과학교실
2단국대학교 의과대학 해부학교실
Correspondence: 
Young Pyo Chang, Email: ychang@dankook.ac.kr
Abstract
Purpose
: To investigate whether growth hormone (GH) has a protective effect on neurons in hippocampal slice cultures of neonatal rats exposed to oxygen-glucose deprivation (OGD).
Methods
: Cultured hippocampal slices of 7-day-old rats were exposed to OGD for 60 min. Then, the slices were immediately treated with three doses of GH (5, 50, or 500 µM) in media. The relative fluorescent densities of propidium iodide (PI) uptake in the slices and relative lactate dehydrogenase (LDH) activities in the media were determined and compared between each GH- treated group of slices and untreated slices (control) at 12 and 24 h after OGD. Immunofluorescent staining for caspase-3 and TUNEL staining were performed to observe the effect of GH on apoptotic neuronal death.
Results
: The relative fluorescent densities of PI uptake in CA1 and dentate gyrus (DG) of the hippocampal slices in each GH-treated group were not significantly different from those in the untreated slices at 12 and 24 h after OGD (P>0.05). Treatment with GH could reduce the relative LDH activities in the media of the GH-treated groups only at 12 h after OGD (P<0.05). Expression of caspase-3 and TUNEL positivity in CA1 and DG of the slices treated with 50-ìM GH were not different from those of the untreated slices at 12 and 24 h after OGD.
Conclusion
: Treatment of hippocampal slice cultures with GH after OGD does not show a definitive protective effect on neuronal death but can reduce the LDH efflux of the slices in media at 12 h after OGD.
Key Words: Growth hormone, Cultured hippocampal slice, Oxygen glucose deprivation, Apoptosis, Neurons


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