| Effects of electromagnetic stimulation on neurogenesis and neuronal proliferation in rat hippocampal slice culture |
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Deok-Soo Kim1, Eung Sang Choi2, Soo Ahn Chae2 |
1Department of Pediatrics, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine
2Department of Pediatrics, College of Medicine, Chung-Ang University, Seoul, Korea |
| 실험 쥐 해마조직배양에서 전자기 자극이 신경조직발생 및 증식에 미치는 영향 |
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김덕수1, 최응상2, 채수안2 |
1성균관대학교 의과대학 강북삼성병원 소아과
2중앙대학교 의과대학 소아과학교실 |
Correspondence:
Soo Ahn Chae, Email: kidbrain@korea.com
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| Abstract |
Purpose
: Transcranial electromagnetic stimulation(TMS) is a noninvasive method which stimulates the central nervous system through pulsed magnetic fields without direct effect on the neurons. Although the neurobiologic mechanisms of magnetic stimulation are unknown, the effects on the brain are variable according to the diverse stimulation protocols. This study aims to observe the effect of the magnetic stimulation with two different stimulation methods on the cultured hippocampal slices.
Methods
: We obtained brains from 8-days-old Spague-Dawley rats and dissected the hippocampal tissue under the microscope. Then we chopped the tissue into 450 µm thickness slices and cultured the hippocampal tissue by Stoppini's method. We divided the inserts, which contained five healthy cultured hippocampal slices respectively, into magnetic stimulation groups and a control group. To compare the different effects according to the frequency of magnetic stimulation, stimulation was done every three days from five days in vitro at 0.67 Hz in the low stimulation group and at 50 Hz in the high stimulation group. After N-methyl-D-aspartate exposure to the hippocampal slices at 14 days in vitro, magnetic stimulation was done every three days in one and was not done in another group. To evaluate the neuronal activity after magnetic stimulation, the NeuN/β-actin ratio was calculated after western blotting in each group.
Results
: The expression of NeuN in the magnetic stimulation group was stronger than that of the control group, especially in the high frequency stimulation group. After N-methyl-D-aspartate exposure to hippocampal slices, the expression of NeuN in the magnetic stimulation group was similar to that of the control group, whereas the expression in the magnetic non-stimulation group was lower than that of the control group.
Conclusion
: We suggest that magnetic stimulation increases the neuronal activity in cultured hippocamal slices, in proportion to the stimulating frequency, and has a neuroprotective effect on neuronal damage. |
| Key Words:
Electromagnetic stimulation , Hippocampal slice culture , NeuN expression , N-methyl-D- aspartate |
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