Korean Journal of Pediatrics 2008;51(1):54-61.
Published online January 15, 2008.
Effects of puromycin aminonucleoside on the cytoskeletal changes of glomerular epithelial cells
Jun Ho Lee1, Tae Sun Ha2
1Department of Pediatrics, Pochon CHA University, College of Medicine
2Department of Pediatrics, Chungbuk National University, College of Medicine
Puromycin aminonucleoside의 사구체 상피세포에 대한 영향
이준호1, 하태선2
1포천중문의대 분당차병원 소아청소년과
2충북대학교 의과대학 소아과학교실
Correspondence: 
Tae Sun Ha, Email: tsha@chungbuk.ac.kr
Abstract
of microvilli, but also separated the intercellular gaps and linear ZO-1. PAN induced oxidative stresses in time and dose dependent manners and increases of intercellular permeability in anti-oxidants inhibitable manners. High concentration of PAN induced not only actin polymerization and disorganization, but also the conglomerulation and internal dislocation of α-actinin protein. The intensities of fluorescences of ZO-1 protein were diminished and internalized by PAN in a dose-dependent manner, which were inhibited by anti anti-oxidants.
Conclusion
: PAN induced the changes of podocytes cytoskeleton and junctional barriers by way of increasing ROS in GEpC that resulted in increasing their permeability in a antioxidatn-inhibitable manner. Glomerular hyperpermeability induced by PAN mediateing through oxidative stresses is thought to take part in the mechanism of proteinuria in nephrotic syndrome.
Key Words: Puromycin aminoglycoside, Nephrotic syndrome, Proteinuria, Oxidative stresses, Anti- oxidants


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