| Plasma G-CSF and GM-CSF Concentrations and Expression of their Receptors on the Granulocyte in Children with Leukocytosis |
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Won Seok Choi, Kyung Hwan Ryu, You Jeong Kim, So Young Kim, Hyun Hee Kim, Wonbae Lee |
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Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea |
| 백혈구 증가증 환아의 혈장내 G-CSF와 GM-CSF의 농도 및 과립구에서의 이들 수용체의 발현 |
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최원석, 유경환, 김유정, 김소영, 김현희, 이원배 |
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가톨릭대학교 의과대학 소아과학교실 |
Correspondence:
Wonbae Lee, Email: lwb@hfh.cuk.ac.kr
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| Abstract |
Purpose
: Granulocyte-colony stimulating factor(G-CSF) and granulocyte macrophage-colony stimulating factor(GM-CSF) are principal cytokines in granulopoiesis and their physiologic effects are mediated through binding to specific cell surface receptors. Although it is known that the level of serum G-CSF and GM-CSF, and presentation of the receptors are increased in infectious diseases, there have been no studies to find the correlation between the granulopoiesis and leukocytosis. This study was designed to measure G-CSF and GM-CSF in leukocytosis and in control and to demonstrate the possible pathogenesis of granulopoiesis in leukocytosis using quantitative analysis of G- CSF, GM-CSF and their CSFr.
Methods
: The plasma levels of G-CSF, GM-CSF of 13 children without leukocytosis and 14 children with leukocytosis were measured. Counts of cell surface G-CSFr and GM-CSFr were measured by combining anti G-CSFr and anti GM-CSFr monoclonal antibodies to their respective receptors by using quantitative flow cytometric assay.
Results
: There was no significant difference betweeen the plasma concentration of G-CSF and GM-CSF in acute leukocytosis and in the control group. However, levels of G-CSFr in acute leukocytosis decreased significantly compared to the control(P=0.012) and the levels of GM-CSFr in both groups revealed no significant difference.
Conclusion
: Increase in the number of leukocyte in leukocytosis was mediated by increasing the number of neutrophil, and increased plasma concentration of G-CSF may be the cause of neutrophilia. But GM-CSF did not have any influence on leukocytosis. |
| Key Words:
Granulocyte-colony stimulating factor(G-CSF), Granulocyte macrophage-colony stimulating factor(GM-CSF), G-CSF receptor, GM-CSF receptor, Leukocytosis |
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