Journal of the Korean Pediatric Society 2001;44(12):1432-1440.
Published online December 15, 2001.
The Immunohistochemical Expression of Neuronal Nitric Oxide Synthase in Rat Hippocampus after Pentylenetetrazole-Induced Seizures
Doo-Kwun Kim1, Tae-Jung Jang2
1Department of Pediatrics, College of Medicine, Dongguk University, Kyongju, Korea
2Department of Pathology, College of Medicine, Dongguk University, Kyongju, Korea
Pentylenetetrazole에 의하여 유발된 경련에서 백서 해마부의 Neuronal Nitric Oxide Synthase에 대한 면역조직화학적 발현
김두권1, 장태정2
1동국대학교 의과대학 소아과학교실
2동국대학교 의과대학 병리학교실
Correspondence: 
Doo-Kwun Kim, Email: pedepi@dumc.or.kr
Abstract
Purpose
: In order to determine the effect of neuronal nitric oxide synthase(nNOS) in seizure-related neuronal vulnerability of the hippocampus, the expression patterns of nNOS were examined in pentylenetetrazole(PTZ)-induced seizure groups and in PTZ seizure groups which were pretreated with nNOS inhibitors.
Methods
: Male Sprague-Dawley rats weighing 200-300 g were used in PTZ(40 mg/kg)-induced seizure experiments. A specific inhibitor, 50 mg/kg 7-nitroindazole(7-NI), and a non-specific inhibitor, 50 mg/kg nitro-L-arginine(L-NA) were treated 30 min before the administration of PTZ to block nNOS. nNOS expression was evaluated by using immunohistochemical staining in the hippocampus of each group.
Results
: The onset time of the first myoclonic jerk was markedly delayed in the 7-NI and the LNA pretreated groups in comparison to the PTZ group. In addition, 7-NI markedly suppressed the severity of PTZ-induced seizures. The expression of nNOS in the hippocampal CA3 area was higher than that in CA1 area in the PTZ treated groups. In the L-NA pretreated groups, the expression levels in the CA3 and CA1 areas were lower than those of the PTZ treated groups. Interestingly, in the 7-NI pretreated groups, the nNOS expression levels in CA1 and CA3 areas were makedly lower than those of PTZ and L-NA pretreated groups. There was no expression in CA2 area of all groups.
Conclusion
: These results suggest that the hippocampal neurons expressing nNOS may be vulnerable to PTZ-induced seizures and that nNOS may play an important role in seizure-related neuronal vulnerability.
Key Words: Neuronal nitric oxide synthase, NOS inhibitor, Immunohistochemistry, Pentylenetetrazole, trazole, Seizure, Rat


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