Journal of the Korean Pediatric Society 1972;15(8):752-757.
Published online August 31, 1972.
A Study of Factors Preparing Tetanus Toxin Sensitized Sheep Red Blood Cells
Woo-Hyun Chang1, Seung Hoon Lee1, Juong Kil Lee2, Hahn Woong Choe2
1Department of Microbiology, College of Medicine, Seoul National University
2Department of Pediatrics, College of Medicine, Seoul National University
破傷風 毒素感作赤血球 製造에 關한 硏究
張友鉉1, 李 承薰1, 李貞吉2, 崔 漢雄2
1서울大學校 醫科大學 微生物學敎室
2서울大學校 醫科大學 小兒科學敎室
Abstract
The passive hemagglutination test first described by Boyden is a most sensitive and convenient method for the measurement of humoral antibodies. Many workers attempted to determine the optimum conditions for preparing red cells sensitized with various antigens. The present paper describes experiments undertaken to find the optimum conditions for coating fresh sheep red cells with partially purified tetanus toxins for measuring minute amount of tetanus antitoxins. The optimum conditions for preparing sheep red blood cells sensitized with tetanus toxin were found as follows. 1. The optimum pH was 6.8 and 7.2. 2. The concentration of Tannic acid was 1 : 30, 000 and 1 : 40, 000. 3. The amount of toxin was 50Lf for 2.5 sheep red cell suspension and 100 Lf for 5% sheep red cell suspension. 4. The concentration of normal rabbit serum as a stabilizer was 1 : 100 dilution. 5. The sensitizing period was 10 min and/or 30 min at both 37°C and 22°C.




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