| Detection of genetic mutations associated with macrolide resistance of Mycoplasma pneumoniae |
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Chi Eun Oh, Eun Hwa Choi, Hoan Jong Lee |
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Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea |
| Mycoplasma pneumoniae의 macrolide 내성과 연관된 유전자 변이의 검출 |
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오지은, 최은화, 이환종 |
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서울대학교 의과대학 소아과학교실 |
Correspondence:
Hoan Jong Lee, Tel: +82.31-787-7283, Fax: +82.31-717-7283, Email: eunchoi@snu.ac.kr
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| Abstract |
Purpose
: The aim of this study was to identify mutations associated with macrolide resistance in Mycoplasma pneumoniae (MP) and to establish a cultural method to determine antimicrobial susceptibility.
Methods
: Nasopharyngeal aspirates (NPAs) were collected from 62 children diagnosed with MP pneumonia by a serologic method or polymerase chain reaction. The 23S rRNA and L4 ribosomal protein genes of MP were amplified and sequenced. To identify mutations in these 2 genes, their nucleotide sequences were compared to those of the reference strain M129. MP cultivation was carried out for 32 (28 frozen and 5 refrigerated) NPAs and M129 strain using Chanock’s glucose broth and agar plate in a 5% CO2 incubator at 37℃ and examined at 2-3 day intervals for 6 weeks.
Results
: Among the 62 specimens, 17 had M144V mutations in ribosomal protein L4. The A2064G mutation was observed in 1 specimen; its 23S rRNA gene was successfully sequenced. Culture for MP was successful from the M129 strain and 2 of the 5 NPAs that were refrigerated for no longer than 3 days. However, MP did not grow from the 28 NPAs that were kept frozen at -80℃ since 2003.
Conclusion
: We found the M144V mutation of L4 protein to be common and that of domain V of 23S rRNA gene was relatively rare among MP. Studies on the prevalence of macrolide-resistant MP and the relationship between the mutations of 23S rRNA gene and ribosomal protein L4 will aid in understanding the mechanism of macrolide resistance in MP. |
| Key Words:
Mycoplasma pneumoniae, Macrolides, Resistance, Mutation |
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