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Toll-like receptor 9 expression and interferon-α secretion upon CpG-ODN stimulation in allergic subjects

Korean Journal of Pediatrics 2009;52(9):1015-1020.
Published online September 15, 2009.
Toll-like receptor 9 expression and interferon-α secretion upon CpG-ODN stimulation in allergic subjects
Man Yong Han1, Hye Mi Jee1, Hyeong Yoon Kim1, Cho Ae Lee1, Hyo-Jin Cho2, Seong-Gyu Hwang2, Kyu-Earn Kim3
1Departments of Pediatrics, CHA University School of Medicine, Seongnam, Korea
2Departments of Internal Medicine, CHA University School of Medicine, Seongnam, Korea
3Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea
알레르기 환자에서 TLR9 ligand인 CpG-ODN 자극에 의한 IFN-α 분비와 TLR9 발현
한만용1, 지혜미1, 김형윤1, 이초애1, 조효진2, 황성규2, 김규언3
1차 의과학대학교 소아과학교실
2차 의과학대학교 내과학교실
3연세대학교 의과대학 소아과학교실
Correspondence: 
Kyu-Earn Kim, Email: kekim@yuhs.ac
Abstract
Purpose
The aim of this study is to explore the effect of the Toll-like receptor 9 (TLR9) expressed in plasmacytoid dendritic cells (pDCs) that respond to antigen to Th2 immune deviation in allergic patients.
Methods
Subjects consisted of 19 allergic patients and 17 healthy volunteers. Skin prick tests and nasal provocation tests were performed for the two groups. Peripheral blood mononuclear cells (PBMCs) were collected from subjects and analyzed for the Lineage Cocktail (CD3, CD14, CD16, CD19, CD20, CD56) (-), HLA-DR (+), and CD123 (+) using flow cytometry. In addition, we analyzed TLR9 mRNA by reverse transcriptase-polymerase chain reaction. The level of interferon-α (IFN-α) of the PBMCs following stimulation with the TLR9 ligand CpG-ODN 2216 was also evaluated.
Results
Analyses of CD123 (+) revealed a nearly similar distribution for the classical pDC markers in the allergic group (0.1%±0.04%) and in the controls (0.25%±0.23%). The mRNA levels of TLR9 on PBMCs were not different between the allergic group and the controls (1.29±0.41 vs. 1.25±0.23, respectively). Additionally, the level of IFN-α in PBMCs exposed to stimuli of the TLR9 ligand CpG-ODN 2216 was not significantly different between the two groups (911±829 vs. 1,095± 888 pg/mL, respectively).
Conclusion
We found no evidence that TLR9-dependent immune responses in human pDCs are associated with allergic status.
Key Words: Allergy, Toll-like receptor 9, Plasmacytoid dendritic cell, Interferon-alpha


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