Korean Journal of Pediatrics 2009;52(5):567-575.
Published online May 15, 2009.
Proteomic analysis of human serum from patients with temporal lobe epilepsy
Chang Woo Lee1, Seung Taek Yu1, Ha Young Choi2, Bun Jeong Koh2, Yong Guen Kwak2
1Department of Pediatrics, Wonkwang University College of Medicine, Iksan, Korea
2Department of Neurosurgery, Chonbuk National University Medical School, Jeonbuk, Korea
측두엽 간질환자의 혈청에서 프로테오믹스기법을 활용한 질병관련 단백질 동정
이창우1, 유승택1, 최하영2, 고은정2, 곽용근2
1원광대학교 의과대학 소아과학교실
2전북대학교 의학전문대학원 신경외과학교실
Correspondence: 
Yong Guen Kwak, Email: ygkwak@chonbuk.ac.kr
Abstract
Purpose
: Epilepsy affects more than 0.5% of the world's population. It has a large genetic component and is caused by electrical hyperexcitability in the central nervous system. Despite its prevalence, the disease lacks definitive diagnostic serological biomarkers. To identify potential biomarkers for epilepsy by a convenient method, we analyzed the expression of serum proteins, reflecting alterations in the patient's proteomes.
Methods
: We compared two-dimensional electrophoretic band patterns of human sera from eight patients with temporal lobe epilepsy (TLE) with those of eight control subjects. The differentially expressed bands were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry.
Results
: Twelve proteins were differentially expressed in the TLE group, of which 6 were identified. Expression of haptoglobin Hp2, PRO2675, immunoglobulin heavy chain constant region gamma 2, an unnamed protein, and three unidentified proteins were upregulated in serum from the patients with TLE, whereas those of major histocompatibility complex (MHC) class I antigen, plasma retinol-binding protein precursor, and three unidentified proteins were downregulated in these patients. After resection of the epileptogenic zone, the expressions of MHC class I antigen, immunoglobulin heavy chain constant region gamma 2, two of the downregulated unidentified proteins, and one of the upregulated unidentified proteins returned to the normal range.
Conclusion
: The 12 serum proteins in this study are potentially useful biomarkers for the diagnosis and monitoring of TLE.
Key Words: Proteomics, Proteome, Epilepsy, Temporal Lobe


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