| Neuroprotective effects of resveratrol via anti-apoptosis on
hypoxic-ischemic brain injury in neonatal rats |
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Jin Young Shin1, Min Ae Seo1, Eun Jin Choi1, Jin Kyung Kim1, Eok Su Seo2, Jun Hwa Lee3, Hai Lee Chung1, Woo Taek Kim1 |
1Department of Pediatrics, School of Medicine, Catholic University of Daegu, Daegu
2Department of Ophthalmology, Dongguk University College of Medicine, Gyeongju
3Department of Pediatrics, Masan Samsung Hospital, School of Medicine, Sungkyunkwan University, Masan, Korea |
| 신생 백서의 저 산소 허혈 뇌손상에서 항세포사멸사를 통한 resveratrol의 신경보호 효과 |
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신진영1, 서민애1, 최은진1, 김진경1, 서억수2, 이준화3, 정혜리1, 김우택1 |
1대구가톨릭대학교 의과대학 소아과학교실
2동국대학교 의과대학 안과학교실
3성균관대학교 의과대학 마산삼성병원 소아청소년과 |
Correspondence:
Woo Taek Kim, Email: wootykim@cu.ac.kr
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| Abstract |
Purpose
: Resveratrol, extracted from red wine and grapes, has an anti-cancer effect, an antiinflammatory effect, and an antioxidative effect mainly in heart disease and also has neuroprotective effects in the adult animal model. No studies for neuroprotective effects during the neonatal periods have been reported. Therefore, we studied the neuroprotective effect of resveratrol on hypoxic-ischemic brain damage in neonatal rats via anti-apoptosis.
Methods
: Embryonic cortical neuronal cell culture of rat brain was performed using pregnant Sprague-Dawley (SD) rats at 18 days of gestation (E18) for the in vitro approach. We injured the cells with hypoxia and administered resveratrol (1, 10, and 30 µg/mL) to the cells at 30 minutes before hypoxic insults. In addition, unilateral carotid artery ligation with hypoxia was induced in 7-day-old neonatal rats for the in vivo approach. We injected resveratrol (30 mg/kg) intraperitoneally into animal models. Real-time PCR and Western blotting were performed to identify the neuroprotective effects of resveratrol through anti-apoptosis.
Results
: In the in vitro approach of hypoxia, the expression of Bax, caspase-3, and the ratio of Bax/Bcl-2, indicators of the level of apoptosis, were significantly increased in the hypoxia group compared to the normoxia group. In the case of the resveratrol-treated group, expression was significantly decreased compared to the hypoxia group. And the results in the in vivo approach were the same as in the in vitro approach.
Conclusion
: The present study demonstrates that resveratrol plays neuroprotective role in hypoxic-ischemic brain damage during neonatal periods through the mechanism of anti-apoptosis. |
| Key Words:
Resveratrol, Hypoxic-ischemic brain injury, Neuroprotective, Newborn, Apoptosis |
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