Transforming growth factor-β promoted vascular endothelial growth factor release by human lung fibroblasts |
Sang Uk Park, Joo Hwa Shin, Jae Won Shim, Deok Soo Kim, Hye Lim Jung, Moon Soo Park, Jung Yeon Shim |
Department of Pediatrics, Sungkyunkwan University School of Medicine, Seoul, Korea |
인간 폐섬유아세포에서 TGF-β 자극에 의한 VEGF 분비 |
박상욱, 신주화, 심재원, 김덕수, 정혜림, 박문수, 심정연 |
성균관대학교 의과대학 강북삼성병원 소아청소년과 |
Correspondence:
Jung Yeon Shim, Email: jyssim@hotmail.com |
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Abstract |
Purpose : The human lung fibroblast may act as an immunomodulatory cell by providing pro-inflammatory cytokines and chemokines, which are important in airway remodeling. Vascular endothelial growth factor (VEGF) induces mucosal edema and angiogenesis. Thymus and activation regulated chemokine (TARC) induces selective migration of T helper 2 cells. We investigated whether human lung fibroblasts produced VEGF and TARC, and the effects were augmented with the co-culture of fibroblasts and human bronchial smooth muscle cells (HBSMC), and whether dexamethasone can inhibit the proliferation and the release of VEGF in lung fibroblasts.
Methods : Human lung fibroblasts were cultured with and without HBSMC, growth-arrested in serum-deprived medium, and pretreated with dexamethasone for 16 hours. After 24-hour stimulation with platelet derived growth factor-BB (PDGF-BB) and/or transforming growth factor-β (TGF-β), culture supernatant was harvested for assays of VEGF and TARC. Cell proliferation was assayed using BrdU cell proliferation ELISA kit.
Results : 1) The release of VEGF was significantly increased after stimulation with TGF-β, and its release was augmented when co-stimulated with PDGF and TGF-β. 2) VEGF release induced by PDGF or TGF-β was inhibited by dexamethasone. 3) There was no synergistic effect on the release of VEGF when human lung fibroblasts were co-cultured with HBSMC. 4) Dexamethasone did not suppress human lung fibroblasts proliferations. 5) Neither TGF-β nor PDGF induced TARC release from lung fibroblasts.
Conclusion : Human lung fibroblasts may modulate airway remodeling by release of VEGF, but they have no synergistic effects when co-cultured with HBSMC. Dexamethasone suppresses VEGF release, not proliferation of lung fibroblast. |
Key Words:
Human lung fibroblast, Transforming growth factor-β, Vascular endothelial growth factor, Thymus and |
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