| Effect of a Flavon Extracted from Artemisia absinthium on Collagen Induced Arthritis in Mice |
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Kyong Min Choi1, Dae Hwan Hwang1, Kyung Mi Shin1, Do-Young Yoon2, Dong Soo Kim1 |
1Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea
2Korea Research Institute of Bioscience and Biotechnology, Cellular Biology Laboratory, Taejeon, Korea |
| Artemisia absinthium (애엽)에서 추출한 Flavon이 마우스에서 Collagen Induced Arthritis에 미치는 영향 |
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최경민1, 황대환1, 신경미1, 윤도영2, 김동수1 |
1연세대학교 의과대학 소아과학교실
2한국과학기술연구소 |
Correspondence:
Dong Soo Kim, Email: dskim6634@yumc.yonsei.ac.kr
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| Abstract |
Purpose
: In this study, a possible suppressive effect of a flavon extracted from Artemisia absinthium on a mouse collagen-induced arthritis (CIA) model was investigated.
Methods
: DBA/1 mice were injected intradermally with emulsified chicken type II collagen. Three weeks after immunization, a flavon was introduced p.o. everyday. Clinical incidences of arthritis and arthritis index were measured. Measurement of anti-collagen antibodies and a stimulation index of the splenocytes of the mice were measured. IL-10 and TNF-α in the supernatants of the mice sera were measured by ELISA. mRNA expression for IL-10 and TNF-α in the splenocytes were tested.
Results
: Flavon extracted from Artemisia absinthium appears to be an effective suppressor of CIA in mice. The serum anti-collagen antibody level and stimulation index of the cultured splenocytes showed no significant differences among the three experimental groups. Also serum IL-10 and TNF-α levels did not show any significant differences among the three experimental groups. An increased expression of mRNA for IL-10 was observed in the splenocytes treated with flavon.
Conclusion
: With these results, flavon extracted from Artemisia absinthium appears to have a suppressive effect of CIA. The mechanism of the suppressive effect of flavon extracted from Artemisia absinthium may be from a stimulation of IL-10 production. |
| Key Words:
Flavon, Artemisia absinthium, Collagen induced arthritis, IL-10, TNF-α |
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