| Hypoxia Induced Expression of Vascular Endothelial Growth Factor in Rat Pulmonary Artery Smooth Muscle Cells |
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Un Seok Nho1, Yeo Hyang Kim2, Myung Chul Hyun2, Sang Bum Lee2 |
1Department of Pediatrics, School of Medicine, The Catholic University of Korea, Daegu, Korea
2Department of Pediatrics, College of Medicine, Kyungpook National University, Daegu, Korea |
| 쥐의 폐동맥 평활근 세포에서 저산소에 의한 Vascular Endothelial Growth Factor의 발현 |
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노은석1, 김여향2, 현명철2, 이상범2 |
1대구가톨릭대학교 의과대학 소아과학교실
2경북대학교 의과대학 소아과학교실 |
Correspondence:
Sang Bum Lee, Email: leesb@knu.ac.kr
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| Abstract |
5% CO2-95% air atmosphere. The smooth muscle cells were confirmed by immunostaining with smooth muscle myosin and α-smooth muscle actin antibodies. The VEGF gene expression in the hypoxic group was compared with the one in control the group as well as the one in the starved group by RT-PCR and Northern blot hybridization.
Results
: There was no statistically significant difference among the control, hypoxic and starved groups.
Conclusion
: There are few studies of pulmonary vascular hypertension at the molecular level in Korea. Therefore, we studied the expression of VEGF gene in hypoxic pulmonary vascular smooth muscle cells. Further studies will be needed to find the difference between newly born and adult rats, or human and rat pulmonary vascular smooth muscle cells in gene expression. We hope that the study will lead to a better understanding of pulmonary vascular hypertension. |
| Key Words:
Hypoxia, Vascular endothelial growth factor, Pulmonary artery smooth muscle cells, RT-PCR, Northern blot hybridization |
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