Effect of Resuscitation with High Concentration Oxygen on a
Rat Model of Neonatal Hypoxic-Ischemic Brain Injury |
Dong Seok Lee1, Su-Hee Kwak1, Heng-Mi Kim1, Yoon Kyung Sohn2 |
1Department of Pediatrics, College of Medicine, Dongguk University, Kyongju, Korea 1Department of Pediatrics, College of Medicine, Kyungpook University, Taegu, Korea 2Department of Pathology, College of Medicine, Kyungpook University, Taegu, Korea |
신생쥐 저산소성 허혈성 뇌손상에 고농도 산소 소생술이 미치는 영향 |
이동석1, 곽수희1, 김행미1, 손윤경2 |
1동국대학교 의과대학 소아과학교실 1경북대학교 의과대학 소아과학교실 2경북대학교 의과대학 병리학교실 |
Correspondence:
Heng-Mi Kim, Email: hmkim@knu.ac.kr or @knuh.knu.ac.kr |
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Abstract |
Purpose : This study was undertaken to determine whether any features of apoptosis would occur in the established model of cerebral hypoxia-ischemia in neonatal rats. It was also undertaken to evaluate the effect of post-insult hyperoxia on hypoxic ischemic cerebral injury.
Methods : Seven-day-old neonatal rats underwent unilateral carotid artery dissection followed by 2 hours of hypoxia. To this end rat pups, allocated into 2 groups, were resuscitated with high concentration O2(>FiO2 95%) or room air for a 1-hour period. All of them were killed at 3 days after the above procedures. Their brains were perfusion fixed and removed to examine tissue damage by light microscope and apoptosis by terminal deoxynucleotidyl transferase mediated dUTP- biotin nick end labeling(TUNEL) reactivity.
Results : The result demonstrates that hypoxia-ischemia model induces tissue damage and TUNEL. Post-insult exposure to high reactivity concentration O2 does not aggravate hypoxic-ischemic cerebral injury 3 days after the insult but increases TUNEL reactivity in injured tissue.
Conclusion : These findings suggest that many cells die by apoptosis following hypoxia-ischemia in neonatal brain and resuscitation with high concentration O2 seems to provide an adverse effect over a brain injury by induction of apoptosis. |
Key Words:
Hypoxia, Ischemia, Newborn rat, TUNEL, Hyperoxia, Resuscitation |
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