Journal of the Korean Pediatric Society 2001;44(7):741-751.
Published online July 15, 2001.
Study on Hepatitis B Virus Pre-S/S Gene Mutations in Children with Chronic Hepatitis B Infection
Byeong Hee Son1, Yeong Hong Park2, Woo Yeong Chung3
1Department of Pediatrics, St. Benedict Hospital, Pusan, Korea
2Department of Biochemistry, College of Medicine, Inje University, Pusan, Korea
3Department of Pediatrics, College of Medicine, Inje University, Pusan, Korea
소아 만성 B형 간염에서 Pre-S/S 유전자의 변이종에 대한 연구
손병희1, 박영홍2, 정우영3
1성분도병원 소아과
2인제대학교 의과대학 생화학교실
3인제대학교 의과대학 소아과학교실
Abstract
Purpose
: Hepatitis B virus(HBV) with various mutations has been reported. The aims of this study were to investigate the frequency and manifestation of HBV pre-S/S mutations in children with chronic hepatitis B infection.
Methods
: Sera from 17 children with chronic hepatitis B infection were analyzed by direct sequencing of polymerase chain reaction amplification of HBV DNA.
Results
: Seventeen cases of adr type were analyzed. The deletions in HBV pre-S region were observed in 3(17.6%) of 17 cases. Of 3 deleted cases, 2 had an in-phase deletion in the pre-S1 region spanning 18 bp. Another case had a 18 bp and 3 bp deletions in the pre-S1 region. Many point mutations in HBV pre-S region were detected in all cases and these mutations were observed more frequently in the pre-S2 region than the pre-S1 region. Six point mutations in the pre-S1 region were observed. Eight point mutations in pre-S2 region were observed. Point mutations in the S region were detected in 14(82.4%) of 17 cases. Among these, mutations of the “a” determinant were detected in 4(23.5%) of 17 cases. Mutations at codon 130 and at codon 146 were noted in 2 cases. Combined mutations at codon 124, 126, 146 and at 130, 131, 136, 146 were noted in the other 2 cases. Mutations except “a” determinant region included at codon 3, 29, 73, 120, 184, 214, 226, 227.
Conclusion
: These observations suggest that deletion and point mutations in HBV pre-S1, pre- S2 regions and point mutations in HBV S region are frequent in the children with chronic hepatitis B infection.
Key Words: Hepatitis B virus, Deletion, Point mutation, Pre-S1 and pre-S2 region, S region, Children


METRICS Graph View
  • 2,014 View
  • 11 Download


Close layer
prev next