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Apoptosis of Cord Blood Mononuclear Cells Infected with Enterovirus

Journal of the Korean Pediatric Society 2000;43(3):372-379.
Published online March 15, 2000.
Apoptosis of Cord Blood Mononuclear Cells Infected with Enterovirus
Dong Soo Kim
Department of Pediatrics, College of Medicine, Yonsei University, Seoul, Korea
장바이러스에 의한 제대혈액 단핵구의 Apoptosis에 관한 연구
김동수
연세대학교 의과대학 소아과학교실
Abstract
Purpose
: To evaluate apoptosis of cord blood mononuclear cells(CBMC) infected with enterovirus.
Methods
: Cord blood was obtained from 20 neonates which were delivered vaginally without any complications. Cord blood mononuclear cells(CBMC) were isolated, and after 24h and 48h incubation, cells were stained with Annexin V and Propidium Iodide and were analyzed with FACScan flowcytometer. CBMCs were also incubated with enterovirus and γ-INF. mRNA expression of FasL was evaluated with RT-PCR.
Results
: The mean rate of apoptosis of CBMC 24 hr after culture with virus showed no definite increase compared to that of culture only group. The mean rate of apoptosis in the culture group with γ-INF was higher than that of culture with virus group(P<0.01) but was not different from the group cultured with virus and γ-INF simultaneously. These findings were also seen in 48- hours-cultured groups. mRNA of CBMC of the groups cultured with γ-INF expressed FasL.
Conclusion
: The upregulated apoptosis of peripheral lymphocytes in patients with aseptic meningitis may be not due to enterovirus infection directly, but due to some mediators such as γ-INF from immune cells released through enteroviral infection.
Key Words: Apoptosis, Enterovirus, Fas-FasL


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