Journal of the Korean Pediatric Society 1999;42(5):686-696.
Published online May 15, 1999.
Effects of Thioredoxin on Oxidative Neuronal Cell Injury
Hyunmi Kim1, Gyoung Hee Kim2, Yoon Song Lee3
1Department of Pediatrics, Ulsan University, College of Medicine, Ulsan, Korea
2Department of Pediatrics, Ewha Womans University, College of Medicine, Seoul, Korea
3Department of Pharmacology†, Sungkunkwan University, College of Medicine, Seoul, Korea
신경세포의 산화적 손상에 대한 Thioredoxin의 영향
김현미1, 김경희2, 이윤송3
1울산대학교 소아과학교실
2이화여자대학교 소아과학교실
3성균관대학교 약리학교실
Correspondence: 
Hyunmi Kim, Email: 1
Abstract
Purpose
: Thioredoxin is an endogenous antioxidant which directly scavenges reactive oxygen species(ROS) and regenerates oxidatively damaged protein by reducing potential at the redox active disulfide(-Cys-Gly-Pro-Cys-) site. Under oxidative stress, thiredoxin plays a protective and adaptative role by inducing expressions. The aim of this study was to clarify the role of thioredoxin on oxidative neuronal cell injury. We investigated the protective effects of E. coli thioredoxin, also acting as a substrate for mammalian thioredoxin reductase, against oxidative neuronal cell injury under oxidative stresses such as hydrogen peroxide and diamide.
Methods
: PC 12 cells were cultured in RPMI 1640 media containing 10% fetal calf serum and subcultured in 96-well plates. Each well contained 30,000 cells. Cells were treated with hydrogen peroxide or diamide 30 minutes after thioredoxin treatment and then incubated for 24 hours. Cytotoxicity and cellular viability were assessed by measuring of lactate dehydrogenase(LDH) release and MTT reduction.
Results
: Thioredoxin not only decreased the cytotoxicity of PC 12 cell treated with hydrogen peroxide by decreasing LDH release and preventing the decrease of MTT reduction but also thioredoxin showed greater protective effects when simultaneously treated with hydrogen peroxide. Also, thioredoxin decreased cytotoxicity by decreasing LDH release from PC 12 cells damaged by diamide. Thioredoxin did not prevent the decrease of MTT reduction on PC 12 cells damaged by diamide.
Conclusion
: Thioredoxin protected PC 12 cells under oxidative stresses by directly scavenging and inhibiting oxidants such as hydrogen peroxide and diamide.
Key Words: Thioredoxin, Oxidative stress, Hydrogen peroxide, Diamide


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