Journal of the Korean Pediatric Society 1999;42(4):553-560.
Published online April 15, 1999.
Immunohistochemical Expression of c-fos Protein and Histologic Findings after Instillation of Kainic Acid in Hippocampus of Neonatal Rat Brain
Soo Ahn Chae, Yong Soo Kim, Byoung Hoon Yoo, Won Bok Lee
Department of Pediatrics, College of Medicine, Chung-Ang University, Seoul, Korea
신생 쥐 해마부위의 Kainic Acid 투여로 인한 c-fos 단백 발현 양상 및 조직학적 소견에 관한 연구
채수안, 김용수, 유병훈, 이원복
중앙대학교 의과대학 소아과학교실
Correspondence: 
Soo Ahn Chae, Email: 1
Abstract
Purpose
: c-fos is rapidly and transiently induced in the intact CNS by a wide variety of exogenous stimuli that include seizures, glutamate receptor activation, sensory stimulation and stress. In adult animals, systemic KA administration produces limbic seizures that results in c-fos protein expression, irreversible morphological changes and localized neuronal death. So we studied the pattern of c-fos protein expression and histological findings in hippocampal formation, following kainic acid-induced seizures during the postnatal period in the rat.
Methods
: Sprague-Dawley rat pups ranging from 8 to 10 days of age, received kainic acid(KA, 5mg/kg) by intraperitoneal injection. Control rats were injected with normal saline. The rats were perfused and fixed with 4% buffered paraformaldehyde at varying time-intervals after KA injection, the brains were sectioned and immunohistochemically stained for c-fos protein and performed HE staining.
Results
: In the hippocampus, immunohistochemistry showed that c-fos protein expressed at 12 hr after KA injection and disappeared thereafter. c-fos protein expressed in all sectors of the hippocampus but most densely expressed in CA1 and CA3 sectors. Rarely c-fos expression was seen in the granular cell layer of dentate gyrus. There were no histologic changes in the hippocampus at 2 weeks after KA injection.
Conclusion
: c-fos, a proapoptotic gene in adult rats, seemed to have an additive role in neuronal cell adaptation to exogenous stimuli in neonatal rats. As a result, it suggests that the roles of c-fos in neuronal cells after noxious stimuli are different between neonatal and adult rats.
Key Words: Kainic acid, c-fos, Neonatal rat brain


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