| Optimal Number of Blood Cultures and Volume of Blood Needed to Detect Bacteremia in Children |
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Jong Jae Kim, Cheol Am Kim, Sang Ho Baik, Eui Tak Oh, Hong Ja Kang, Kil Seo Kim |
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Department of Pediatrics, Dae Dong Hospital, Pusan, Korea |
| 소아에서 균혈증을 진단하기 위해 필요한 혈액 배양 검사의 적절한 횟수와 채혈량 |
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김종재, 김철암, 백상호, 오의탁, 강홍자, 김길서 |
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대동병원 소아과 |
Correspondence:
Jong Jae Kim, Email: 1
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| Abstract |
Purpose
: We compared pathogen recovery rates by obtaining two blood cultures instead of one blood culture containing 1ml and collecting a larger volume, 1 to 3ml.
Methods
: Total of 750 blood specimens from 250 patients with fever, a temperature higher than 39℃ and suspected bacteremia were obtained. Each patient had two samples of blood, A(1ml) and B(4ml), obtained at 30-minute interval from separate sites of extremities and B was divided into B1(1ml) and B2(3ml). Each sample was inoculated into aerobic culture media. Patients were excluded if two samples of blood were not obtained or if the isolate represented a contaminant.
Results
: A pathogen was isolated in 19(7.6%) of 250 patients and 37(4.9%) of 750 specimens. In 7 patients, the pathogen was isolated with all the culture methods and in 12 patients, one or more of the cultures yielded no growth. The pathogen recovery rates were 53%(10/19) in A and B1, 89%(17/19) in B2 and 68%(13/19) in A＋B1. No difference was detected between A or B1 and A＋B1(P>0.05) and the pathogen recovery rate for B2 was significantly greater than that for A or B1(P<0.05), but no significant differences were found in pathogen recovery when B2 was compared with A＋B1.
Conclusion
: Increasing volume of blood from 1 to 3ml inoculated into blood culture bottles improves detection of bacteremia in pediatric patients and spares patients the cost and pain of an additional venipuncture. |
| Key Words:
Blood culture, Number, Volume |
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