Journal of the Korean Pediatric Society 1998;41(2):154-162.
Published online February 15, 1998.
Developmental mRNA Expression of Cellular Retinoic Acid Binding Protein I and Ⅱ in Rat
Young Yoo1, Hyung Suk Kim2, Chang Sung Son2, Young Chang Tockgo2, Young Hyuk Jeon1
1Department of Pediatrics, Seoul Dongboo Municipal Hospital, Seoul, Korea
2Department of Pediatrics, College of Medicine, Korea University, Seoul, Korea
흰쥐 발달 과정에서의 세포 retinoic acid 결합단백 I과 II의 유전자 발현에 관한 연구
유영1, 김형석2, 손창성2, 독고영창2, 전용혁1
1서울시립동부병원 소아과
2고려대학교 의과대학 소아과학교실
Chang Sung Son, Email: 1
: Retinoic acid(RA) is well known as a potent teratogenic agent in both deficiency and excess. Cellular retinoic acid binding proteins(CRABPs) are involved in RA. We carried this study in order to determine the possible relations of CRABPs with RA-induced teratogenesis through observation of the expression patterns of CRABP Ⅰand Ⅱ in developing rats.
: 35S-labeled RNA probes were synthesized using SP6-RNA polymerase in CRABP Ⅰand T7-polymerase in CRABP Ⅱ. The distribution of CRABP Ⅰand Ⅱ transcripts analyzed by in situ hybridization of rat embryosections from day 12 to 19, and postnatal brains from day 1 to 14.
: The CRABP Ⅱ transcripts were more widely distributed than CRABPⅠdistribution, however, the relative level of CRABPⅠ transcripts were higher than CRABP Ⅱ. The CRABP Ⅰ mRNA transcripts showed its highest expression on the 16th day of gestation and these distribution correlated well with structures known to be targets of RA-induced teratogenesis. CRABP Ⅱ transcripts were expressed in brain vesicle, spinal cord, head, face, tongue and genital tubercle and also found in the structures which are not involved in RA-induced teratogenesis.
: These results suggest the possible involvement of both CRABPs in the RA-induced teratogenesis. However, CRABP Ⅰ may have more specific roles than CRABP Ⅱ which may play a role through a different mechanism.
Key Words: CRABP Ⅰ, CRABP Ⅱ, Rat Development, In situ hybridization, Teratogenesis

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