Journal of the Korean Pediatric Society 1997;40(9):1265-1274.
Published online September 15, 1997.
Immunohistochemical Study of Bcl-2 Oncoprotein Expression in Childhood Non-Hodgkin's Lymphoma
Sung Mee Kim1, Jin Hwa Jung1, Jeong Ho Lee1, Jong Dae Jo1, Young Tak Lim2, Do Youn Park3, Sun Kyeung Lee3
1Department of Pediatrics, Marynoll Hosipital, Pusan, Korea
2Department of Pediatrics, Pusan National University, Pusan, Korea
3Department of Pathology, Pusan National University, Pusan, Korea
소아의 비 Hodgkin 림프종에서 Bcl-2 암단백 발현의 면역조직화학적 연구
김성미1, 정진화1, 이정호1, 조종대1, 임영탁2, 박도윤3, 이선경3
1부산 메리놀병원 소아과
2부산대학교 의과대학 소아과학교실
3부산대학교 의과대학 병리학교실
Abstract
Purpose
: The aim of the present study was to assess the clinicopatholgical significance of Bcl-2 oncoprotein expression in childhood non-Hodgkin' s lymphoma(NHL).
Methods
: We have assessed 16 cases of childhood NHL during last 6 years from 1990 to 1995. Bcl-2 oncoprotein expression has been semiquantitatively analyzed in paraffin sections from 16 cases of childhood NHL with 39 control cases of adult NHL. The expression of Bcl-2 oncoprotein was correlated with histologic grade, immunophenotype, proliferative activity as measured by immunostain of Ki-67 antigen, clinical stage, and survival rate(event free survival rate, EFS).
Results
: 1) Bcl-2 oncoprotein expression significantly decreased according to increase of histological grade(P < 0.05). 2) High Bcl-2 oncoprotein expression was more frequent in B-cell NHL than T-cell NHL(P < 0.05). 3) Proliferative activity as measured by immunostain of Ki-67 antigen was negatively correlated with Bcl-2 oncoprotein expression(P < 0.05). 4) Bcl-2 oncoprotein expression was not correlated with clinical stage(P > 0.05). 5) EFS of patients was significantly correlated with Bcl-2 oncoprotein expression, that is, reduced EFS was demonstrated in the patients with low Bcl-2 oncoprotein expression (P < 0.05).
Conclusions
: Thus Bcl-2 oncoprotein, as demonstrated immunohistochemically in routinely paraffin embedded tissue, can be restrictively used in prediction of prognosis and grade of childhood NHL, in aggrement with the role of Bcl-2 oncoprotein in blocking of apoptosis and indirect contribution to increase of cellular proliferative activity of NHL.
Key Words: Bcl-2 oncoprotein, Non-Hodgkin’s Lymphoma, Children


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