Warning: fopen(/home/virtual/pediatrics/journal/upload/ip_log/ip_log_2024-03.txt) [function.fopen]: failed to open stream: Permission denied in /home/virtual/pediatrics/journal/ip_info/view_data.php on line 82

Warning: fwrite(): supplied argument is not a valid stream resource in /home/virtual/pediatrics/journal/ip_info/view_data.php on line 83
Rapid Identification of Mycoplasma Pneumoniae, Mycobacterium Tuberculosis and Staphylococcus Aureus in Pleural Fluid by PCR

Journal of the Korean Pediatric Society 1997;40(9):1232-1241.
Published online September 15, 1997.
Rapid Identification of Mycoplasma Pneumoniae, Mycobacterium Tuberculosis and Staphylococcus Aureus in Pleural Fluid by PCR
Chang Wan Kim1, Byung Moon Ahn1, Eun Ryoung Kim1, Il Su Kim2, Yung Seuk Pak3, Sang Chul Sung3
1Department of Pediatrics, Sung-Ae General Hospital, Seoul, Korea
2Sung-Ae Genentic Engeenearing Research Institute
3Department of Molecular Biology, Seoul Medical Science Institute, Seoul, Korea
감염성 늑막염의 늑막삼출액으로부터 중합효소연쇄반응을 이용한 마이코플라즈마균, 결핵균 및 황색 포도상구균의 특이 유전자분석의 임상적 의의
김창완1, 안병문1, 김은령1, 김일수2, 박영석3, 성상철3
1성애병원 소아과
2성애 유전공학 연구소
3서울의과학 연구소
Abstract
Purpose
: Pleural effusions may develop during the course of bacterial pneumonia. The aim of this study was to evaluate the significance of the polymerase chain reaction(PCR) method for detection of M ycoplasma pneumoniae, M ycobaterium tuberculosis and Staphylococcus aureus from pleural fluid.
Methods
: Total 12 samples were obtained from pleural fluid; 2 samples from children with Mycoplasma pneumonia, 5 samples from adults with tuberculous pleurisy, and 5 samples from sterile pleural fluid seeded artificially with staphylococcus aureus. The primers used for our PCR were prepared to amplify M . pneumonia-specific MP5 gene, M. tuberculosis-specific IS6110 gene, and S. aurus-specific femA and mecA gene. The amplified PCR products were detected by ethidium bromide-stained agarose gel electrophoresis.
Results
: A total of 12 pleural fluid samples were tested by nested PCR using the specific primer set. We could amplify MP5 gene in 2 samples, IS6110 gene in 5 samples, mecA gene in 3 samples, and femA gene in 5 samples. These PCR data were correlated with serolological data, microbiological data and methicillin-sensitivity test result. There were no false-positive results due to cross-contaminating DNA between these 3 organisms.
Conclusions
: We conclude that enzymatic amplification of specific gene from pleural fluid might be useful to diagnose the infectious pleural effusion by M ycoplasma pneumoniae, M ycobacterum tuberculosis or Staphylococcus aureus.
Key Words: M ycoplasma pneumoniae, M ycobacterium tuberculosis, Staphylococcus aureus, Pleural effusion, Polymerase chain reaction


METRICS Graph View
  • 2,460 View
  • 64 Download