Journal of the Korean Pediatric Society 1997;40(8):1162-1168.
Published online August 15, 1997.
Acute Renal Failure(ARF) Associated with Yersinia Pseudotuberculosis Infection Diagnosed by Polymerase Chain Reaction
Hyung Gyu Park1, Byung Moon Ahn1, Eun Ryoung Kim1, Il Soo Kim1, Jae Jong Kim2, Sung Ok Kang2, Han Ho Park2, Ki Jeong Kim3
1Department of pediatrics, Sung-Ae General Hospital, Seoul, Korea
2Bioneer Corp. R & D Center
3Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Korea
급성 신부전증이 합병된 Yersinia pseudotuberculosis 감염 환아에서 inv 유전자 검출로 진단한 1례
박형규1, 안병문1, 김은령1, 김일수1, 김재종2, 강성옥2, 박한호2, 김기정3
1성애병원 소아과
2바이오니아 부설 연구소
3중앙대학교 의과대학 미생물학교실
Abstract
The clinical significance of Yersinia pseudotuberculosis(Y. pseudotuberculosis) has recently recognized in various part of the world because it can cause a wide range of clinical problems such as mesenteric lymphadenitis, septicemia, reactive arthritis, terminal ileitis, erythema nodosum and acute renal failure. we experienced a case of acute renal failure associated with Y. pseudotuberculosis infection. We applied a nested polymerase chain reaction method for rapid diagnosis of Y. pseudotuberculosis infection. DNA was extracted from standard strains of Y. pseudotuberculosis, Y. enterocolitica, serial blood samples, urine, and mountain water by phenol-chloroform method. Using specific primers, we amplified inv and ail gene from extracted DNA samples by PCR method. The patient’ serotype was Y. pseudotuberculosis 2a and it􋺴s titer was 1:40 initially, after 2 weeks the titer increased to 1:160. Stool culture was negative. Inv gene amplification was positive in patient's urine, febrile stage blood, and mountain water. The nested polymerase chain reaction method can be used clinically for rapid diagnosis of Y. pseudotuberculosis infection. So we report here the clinical findings and PCR method of this case with brief review of the literatures.
Key Words: Yersinia pseudotuberculosis, Acute renal failure, Polymerase chain reaction


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