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Diagnosis of Cytomegalovirus Infection in Neonatal and Infantile Periods Using Polymerase Chain Reaction

Journal of the Korean Pediatric Society 1996;39(9):1271-1279.
Published online September 15, 1996.
Diagnosis of Cytomegalovirus Infection in Neonatal and Infantile Periods Using Polymerase Chain Reaction
Young Ah Lee, Kyoung Lee, In Seong Jo, Woo Gap Chung
Department of Pediatrics, College of Medicine, Hallym University, Seoul, Korea
중합효소연쇄반응을 이용한 신생아 및 영아기의 거대세포바이러스 감염의 진단
이영아, 이경, 조인성, 정우갑
한림대학교 의과대학 소아과학교실
Abstract
Purpose
: The aims of present study were to document the incidence of cytomegalovirus infection in neonatal and infantile periods especially in high-risk patients of associated infection by nested PCR of DNA from leukocytes and to evaluate the effectiveness of nested PCR in the diagnosis of congenital cytomegalovirus infection.
Methods
: Genomic DNA was extracted from peripheral leukocytes of 204 neonates and infants during the period from June 1994 to March 1995, and nested PCR was performed. The cases were divided into six groups according to clinical profiles; Group IA-healthy fullterm babies, group IB-fullterm babies with suspected or definite perinatal infection, group IIA-healthy preterm babies, group IIB-preterm babies with suspected or definite perinatal infection, group III-infants with clinical features of viral infection and abnormal liver function tests, group IV-IgM seropositive babies with clinical evidence of cytomegalovirus infection.
Results
: In group ⅠA(n=70), 2 cases were positive for PCR amplification (2.9%). All of the cases in group ⅠB(n=64), group ⅡA(n=15), and group ⅡB(n=20) were negative for cytomegalovirus infection. Only one case(3.3%) among group Ⅲ(n=30) showed positive reaction, while all of the five cases(100%) in group Ⅳ(n=5) were positive.
Conclusion
: The incidence of congenital cytomegalovirus infection in our analysis were 1.2% (2/169), and it was similar to that of western countries. Nested PCR was proved to be a both sensitive and specific diagnostic tool in the diagnosis of cytomegalovirus infection and it seems to be useful in the screening of congenital cytomegaloviral infection in the newborn period. Future analysis of samples including non-blood origin from adult women and newborns will be helpful in the epidemiologic analysis of cytomegalovius infection in this period.
Key Words: Congenital cytomegalovirus infection, Nested PCR, Screening


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