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Production, Characterization, and Clinical Application of Perchloric Acid Soluble M. tuberculosis Antigen (TB-I)

Journal of the Korean Pediatric Society 1993;36(8):1107-1115.
Published online August 15, 1993.
Production, Characterization, and Clinical Application of Perchloric Acid Soluble M. tuberculosis Antigen (TB-I)
Kyung Hyo Kim1, John Linton2, Yunsop Chong3, Dong Soo Kim3
1Department of Pediatrics, College of Medicine, Ewha Woman`s University, Seoul, Korea
2Department of Family Medicine, College of Medicine, Yonsei University, Seoul, Korea
3Department of Clinical Pathology, College of Medicine, Yonsei University, Seoul, Korea
3Department of Clinical Pathology, College of Medicine, Yonsei University, Seoul, Korea
활동성 결핵환자에서 Perchloric Acid 가용성 M. tuberculosis항원에 대한 항체 반응(TB-I)
김경효1, 인요한2, 정윤섭3, 김동수3
1이화대학교 의과대학 소아과학교실
2연세대학교 의과대학 가정의학교실
3연세대학교 의과대학 임상병리학교실
3연세대학교 의과대학 소아과학교실
Abstract
The immune response to M. tuberculosis engages T-cell medicated reactions which determine the degree of resistance and also the clinical pattern of disease. Although antibodies produced by the infected host have yet no proven protection, they are appilicable for alternative diagnostic tests in tuberculosis. Preparation of purified antigens from Mycobacteria with specific antigenic determinants would improve serological diagnosis in tuberculosis. The antigen was prepared by extraction of M. tuberculosis with perchloric acid which was found to be major bands of 71, 42, 38 and 10 kD by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thirteen sera from patients with active tuberculosis showed positive reactions to TB-P by ELISA, but the reaction to perchloric acid extract antigen is weaker than to PPD. The IgG subclass profiles to TB-P were IgG1, IgG2 and IgG4 By means of western blotting, the antibodies in the tuberculous patients showed the reaction with antigens of lower molecular weight such as 14, 12, 11 and 10kD. These results suggested that the perchloric acid soluble antigen of M. tuberculosis might be more related with cell-mediated immune reactions rather than humoral immune reactions.
Key Words: M. tuberculosis, Perchloric acid soluble antigen, Tuberculosis


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