Journal of the Korean Pediatric Society 1993;36(4):495-505.
Published online April 15, 1993.
Effect of Cyclosporin, Indomethacin and Methylprednisolone on Puromycin-Aminonucleoside Induced Nephrosis in Rats
Hong Bae Kim1, Hae Lee Chung1, Cheol Woo Ko1, Ja Hoon Ko1, Jung Sik Gwak2
1Department of Pediatrics, School of Medicine, Kyung-pook National University, Taegu, Korea
2Department of Anatomical Pathology, School of Medicine, Kyung-pook National University, Taegu, Korea
Cyclosporin, Indomethacin 및 Methylprednisolone이 Puromycin-Aminonucleoside 투여로 초래된 백서신증에 미치는 영향
김홍배1, 정혜리1, 고철우1, 고철우1, 곽정식2
1경북대학교 의과대학 소아과학교실
2경북대학교 의과대학 병리학교실
Abstract
This experimental study was conducted to evaluate the effect of indomethacin and methylprednisolone on PAN-induced nephrosis in rats. Sprague-Dawley rats weighing 150∼200gm were used and divided into controls, group Ⅰ(PAN intraperitoneally), group Ⅱ(PAN intraperitoneally, followed by indomethacin peritoneally for 12 days), group Ⅲ(PAN intraperitoneally, followed by methylprednisolone peritoneally for 12 days) and group Ⅳ(PAN intraperitoneally, followed by cyclosporin subcutaneously for 12 days). Twenty four-hour urinary protein excretion was measured on day 0, 5, 10 and 17. On the 17th day, rats were sacrificed for the determination of total serum protein, albumin and cholesterol levels. Foot process widths of glomerular epithelial cells were measured, and anionic sites of lamina rara externa were determined by using PEI as cationic probes. The following results were obtained. Twenty four-hour urinary protein excretion(mg/day) of group I was significantly increased to 455. 7±188.8 on the 5th day compared to 15.2±3.7 on day(p<0.01), and increased gradulally to 525. 6±203.5 on the 10th day, then decreased to 280.6±25.2 on the 17th day. In group Ⅲ, 24 hr srinary protein excretion on 17th day(180.7±64.5) was significantly lower than that of group Ⅰ(280.6±25.2). Total serum protein of group Ⅲ was significantly lower than that of group Ⅰ, Ⅱ, Ⅲ and Ⅳ. Foot process widths(nm) of glomerular epithelial cells in group Ⅰ, Ⅱ, Ⅲ and Ⅳ were 409.5±15.2, 387.8±49.2, 279.9±36.9 and 398.3±38.3, respectively. And the value of group Ⅲ was significantly lower than that of group Ⅰ(p<0.01). The number of anionic sites per 1㎛ length of glomerular basement membrane in Group Ⅰ, Ⅱ, Ⅲ and Ⅳ were 10.3±1.3, 10.1±1.6, 12.5±1.5 and 10.2±1.5, respectively. And the value of group Ⅲ was significantly lower than that of group Ⅰ(p<0.01). In conclusion, cyclosporin and indomethacin did not show any significant effect on PAN nephrosis in rat. However, methylprednisolone reduced the urinary protein excretion and showed significant recovery of foot process widths and number of anionic sites of glomerular basement membrane.
Key Words: Puromycin-aminonucleoside, Nephrotic syndrome, Cyclosporin, Indomethacin, Methylpredrisolone


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