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Original Article

The optimal conditions to improve retrovirus-mediated transduction efficiency to NIH 3T3 cells

Jun Ah Lee, Kang-Min Lee, Hyun Jae Lee, Yun Jeong Lee, Dong Ho Kim, Jung Sub Lim, Kyung-Duk Park

Clin Exp Pediatr. 2007;50(10):1011-1017. Published online October 15, 2007

Purpose : We tried to assess the optimal conditions to improve low transduction efficiency and their effect on target cells. Methods : Cultured NIH 3T3 cells were incubated with retroviral vectors bearing an enhanced green fluorescent protein (eGFP) gene. We varied the ratio of viral vectors to target cells (1:1-1:8) and the number of transfections (X1, X2), and compared transduction...

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