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Original Article

The Separate and Combined Effects of Insulin, Dexamethasone and Growth Hormone on the OB Gene Expression and Leptin Secretion from Cultured Human Visceral Adipose Tissue

Il Tae Hwang, Kyung Hee Kim, Jin Soon Hwang, Choong Ho Shin, Sei Won Yang

Clin Exp Pediatr. 2003;46(8):795-802. Published online August 15, 2003

Purpose : We investigated the hormonal control of OB gene expression and leptin secretion in cultured human visceral adipose tissue. Methods : Visceral adipose tissues were cultured for up to 48 hrs in modified Eagle's medium with varying concentration of hormones : Control(no hormone), bovine insulin(100 nM), Dexamethasone(DEX, 100 nM), growth hormone(GH, 40 ng/mL), insulin＋DEX(100 nM each), insulin＋DEX＋GH(100 nM insulin...

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