| The Effect of Tumor Necrosis Factor-α in
Cultured Neonatal Rat Cardiomyocytes |
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Su Jin Kim1, Dong Seok Lee1, Ok Ko2, Il Soo Mun2, Bok Hyun Ko2, Yong Wook Jung2 |
1Department of Pediatrics, College of Medicine, Dongguk University, Kyungju, Korea
2Department of Anatomy, College of Medicine, Dongguk University, Kyungju, Korea |
| 배양된 신생 백서 심근 세포에 대한Tumor Necrosis Factor-α의 효과 |
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김수진1, 이동석1, 고옥2, 문일수2, 고복현2, 정용옥2 |
1동국대학교 의과대학 소아과학교실
2동국대학교 의과대학 해부학교실 |
Correspondence:
Dong Seok Lee, Email: lds117@dumc.or.kr
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| Abstract |
Purpose
: Tumor necrosis factor-α(TNF-α) is a pro-inflammatory cytokine that has been implicated in the pathogenesis of cardiovascular disease. Serum levels of TNF-α are elevated in many human cardiac related pathogenic conditions, including heart failure. It is well known that TNF-α inhibits myocardial contractility and induces apoptosis of adult rat cardiomyocytes via stimulation of TNF receptor 1. But pathophysiologically relevant low levels of TNF-α can not induce apoptosis of neonatal cardiomyocytes. So, we evaluated the effects of different concentrations of TNF-α in cultured rat neonatal cardiomyocytes : apoptosis or necrosis.
Methods
: Neonatal ventricular myocytes were isolated from 3-day-old rats by stepwise collagenase dissociation, and the cells were cultured for 3 days. After that, cardiomyocytes were treated with low(25 ng/mL) and high(250 ng/mL) concentration of TNF-α for 48 hours. Apoptosis was determined by terminal deoxynucleotidyl transfer-mediated end labelling(TUNEL) staining, and cell viability was evaluated by lactate dehydrogenase(LDH) measurements using cell culture supernatants.
Results
: Low dose TNF-α did not induce apoptosis compared with controls(10.5±3.5% : 10.4±4.3%). And high dose TNF-α also did not induce significant apoptosis(10.2±3.6% : 10.4±4.3%). There was no detectable morphological changes of cardiomyocytes after low and high concentration of TNF-α treatment. LDH levels after TNF-α treatment was not significant compared with control(control : low : high, 3.2±0.1% : 3.1±0.2% : 3.3±0.3%).
Conclusion
: Our results suggest that high concentration of TNF-α alone can not induce apoptosis and significant cytotoxicity in neonatal rat cardiomyocytes. |
| Key Words:
TNF-α, Neonatal rat, Cardiomyocyte, Apoptosis |
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