| A Study of Relation between Stable Microbubble Rating and Surfactant Derivatives(SurfactenⓇ : Phospholipid :, ExosurfⓇ : Dipalmitoyl-Phosphatidyl Choline) Concentration in Vitro |
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Myung Ho Oh, Kee Hyuck Kim, Jae Gun Sim, Min Hee Kim |
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Department of Pediatrics, College of Medicine, Kon-Kuk University Hospital, Chungju, Korea |
| 폐 표면 활성제의 농도에 따른 Stable Microbubble Rating 치의 변화에 관한 연구 |
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오명호, 김기혁, 심재건, 김민희 |
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건국대학교 의과대학 소아과학교실 |
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| Abstract |
Purpose
: With the recent advent of surfactant replacement therapy, there is an increasing need
for a rapid and reliable test to predict respiratory distress syndrome (RDS) immediately before or
at birth. There are many investigations and methods for the detection of RDS in prenatal or
postnatal period. The stable microbubble rating test (SMR-test) developed by pattle et al. is rapid
and simple test performed on amniotic fluid and gastric aspirates which reflects the adequacy of
pulmonary surfactant with higher diagnostic accuracy. To determine the relation of the SMR and
surfactant derivatives [SurfactenⓇ : phospholipid (PL), ExosurfⓇ : dipalmitoyl-phosphatidyl choline
(DPPC) concentration in vitro, author performed SMR test according to 15 groups of surfactant
derivatives concentration by using modified Pattle' s method.
Methods
: SurfactenⓇ & ExosurfⓇ were diluted each concentration (0, 5, 10, 15, 20, 25, 30, 35, 40,
50, 60, 70, 80, 90, 100μg/ml) by dilutional fluid. We enveloped test tube by paraffin paper for
protection of evaporation. When we performed SMR test, we shaked test tube vigorously by
Vlotex shaker. We performed SMR test according to 15 groups of Surfactant concentration by
using modified Pattle' s method.
Results
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1) The number of SMR according to 15 groups of surfactant derivatives concentration were 2,
1/mm2 in 4.2μg/ml of PL and 3.1μg/ml of DPPC, 279, 1104/mm2 in 83.3μg/ml of PL and 61.8μg/ml
DPPC.
2) The regression curve of SMR and surfactant derivatives concentration showed statistically
significant relation(p< 0.005).
Conclusion
: The SMR test was a good method in estimation of surfactant concentration in vitro
and also in diagnosis of RDS recognized as a surfactant deficiency. |
| Key Words:
Respiratory distress syndrome, Surfactant, SurfactenⓇ, , ExosurfⓇ, , Stable microbubble rating test |
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