| Detection of Enterovirus in Patients with Aseptic Meningitis by Reverse Transcription and Polymerase Chain Reaction |
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Souck Joong Yoon1, Sung Jin Hong1, Young Hyuk Lee1, Min Hee Kim1, Kyo Sun Kim1, Kyu Hyun Park2, Jae Myun Lee3, Won Young Lee3 |
1Department of Pediatrics, KonKuk University College of Medicine, Seoul, Korea
2Yonsei Cancer Center
3Department of Microbiology,Yonsei University College of Medicine, Seoul, Korea |
| 역전사 중합효소 연쇄반응을 이용한 무균성 뇌막염 바이러스의 진단 |
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윤석중1, 홍성진1, 이영혁1, 김민희1, 김교순1, 박규현2, 이재면3, 이원영3 |
1건국대학교 의과대학 소아과학교실
2연세의료원 암센터
3연세대학교 의과대학 미생물학교실 |
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| Abstract |
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The aim of this study was to determine the applicability of reverse transcription and polymerase chain reaction(RT/PCR) for routine diagnostic use and for the detection of enteroviral mentigitis, Primers were selected in the highly conserved part of the 5'-non-coding region of the enteroviral genome. Enteroviral RNA was detected by the RT/PCR in routinely collected cerebrospinal fluids(CSF) of patients with aseptic menigitis who had admitted to the dept. of Pediatrics, MinJoong Hospital, KonKuk University from May to July 1993. Enteroviral RNA was detected in one third of CSF specimen. Our results demonstrate the significance of the RT/PCR in the diagnosis of enteroviral menigitis. |
| Key Words:
Aseptic meningitis, Reverse transcription and Polymerase Chain Reaction |
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