| Production and Characterization of Monoclonal Antibodies to Perchloric Acid Soluble Antigen of M. tuberculosis(TB-II) |
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Kyung Hyo Kim1, Dong Soo Kim2 |
1Department of Pediatrics, Ewha Woman`s University College of Medicine, Seoul, Korea
2Department of Pediatrics, Yonsei University, College of Medicine, Seoul, Korea |
| Perchloric Acid 분리 M. tuberculosis 항원에 대한 단세포군 항체의 제조 및 분석(TB-II) |
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김경효1, 김동수2 |
1이화대학교 의과대학 소아과학교실
2연세대학교 의과대학 소아과학교실 |
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| Abstract |
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Mycobacteria cause diseases which occur the world over and whoich carry a considerable burden in morbidity, mortality and social problems. A battery of monoclonal antibodies specific for mycobacterial antigens would provide a useful tool for rapid diagnosis of mycobacterial diseases. Fourtenn monoclonal antibodies to perchloric acid soluble antigne of M. tuberculosis were produced. Immunoglobulin isotypes of monoclonal antibodies were ten of immunoglobulin G2a, two of IgG3, and two of IgM. By means of Western blotting, monoclonal antibody detected the antigen of 54 kD in TB-P. In the immunofluorescence assay, the monoclonal antibody showed a positive reaction with intact M. tuberculosis bacilli, M. tuberculosis in the pulmonary tissue of tuberculous patient and M. bovis BCG. |
| Key Words:
M. tuberculosis, Monoclonal antibodies |
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